Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3988049..3988546 Replicon chromosome
Accession NZ_CP104649
Organism Escherichia coli strain PNUSAE008512

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5913_RS20490 Protein ID WP_001173343.1
Coordinates 3988373..3988546 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5913_RS20485 Protein ID WP_000398619.1
Coordinates 3988049..3988345 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5913_RS20465 (3984780) 3984780..3985499 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5913_RS20470 (3985496) 3985496..3987127 - 1632 WP_001216477.1 sensor histidine kinase -
N5913_RS20475 (3987308) 3987308..3987538 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N5913_RS20480 (3987550) 3987550..3987822 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5913_RS20485 (3988049) 3988049..3988345 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5913_RS20490 (3988373) 3988373..3988546 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5913_RS20495 (3988665) 3988665..3990182 - 1518 WP_001295074.1 lysine--tRNA ligase -
N5913_RS20500 (3990419) 3990419..3991876 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258862 WP_001173343.1 NZ_CP104649:3988373-3988546 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258862 WP_000398619.1 NZ_CP104649:3988049-3988345 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References