Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3168509..3169140 Replicon chromosome
Accession NZ_CP104649
Organism Escherichia coli strain PNUSAE008512

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag N5913_RS16660 Protein ID WP_001259385.1
Coordinates 3168865..3169140 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag N5913_RS16655 Protein ID WP_000593555.1
Coordinates 3168509..3168868 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5913_RS16625 (3163621) 3163621..3164094 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
N5913_RS16630 (3164091) 3164091..3164372 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
N5913_RS16635 (3164449) 3164449..3165807 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
N5913_RS16640 (3165800) 3165800..3167308 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
N5913_RS16645 (3167298) 3167298..3167567 + 270 WP_000933060.1 HPr family phosphocarrier protein -
N5913_RS16650 (3167599) 3167599..3168459 + 861 WP_001131788.1 class II aldolase -
N5913_RS16655 (3168509) 3168509..3168868 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N5913_RS16660 (3168865) 3168865..3169140 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
N5913_RS16665 (3169213) 3169213..3170337 - 1125 WP_001301659.1 ABC transporter permease -
N5913_RS16670 (3170337) 3170337..3173072 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
N5913_RS16675 (3173069) 3173069..3174136 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T258859 WP_001259385.1 NZ_CP104649:c3169140-3168865 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT258859 WP_000593555.1 NZ_CP104649:c3168868-3168509 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References