Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2587660..2588314 Replicon chromosome
Accession NZ_CP104649
Organism Escherichia coli strain PNUSAE008512

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag N5913_RS13680 Protein ID WP_000244781.1
Coordinates 2587660..2588067 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N5913_RS13685 Protein ID WP_000354046.1
Coordinates 2588048..2588314 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5913_RS13660 (2583617) 2583617..2585350 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
N5913_RS13665 (2585356) 2585356..2586066 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N5913_RS13670 (2586091) 2586091..2586987 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
N5913_RS13675 (2587099) 2587099..2587620 + 522 WP_001055874.1 flavodoxin FldB -
N5913_RS13680 (2587660) 2587660..2588067 - 408 WP_000244781.1 protein YgfX Toxin
N5913_RS13685 (2588048) 2588048..2588314 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N5913_RS13690 (2588557) 2588557..2589537 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
N5913_RS13695 (2589614) 2589614..2590273 - 660 WP_000250274.1 hemolysin III family protein -
N5913_RS13700 (2590437) 2590437..2590748 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
N5913_RS13705 (2590793) 2590793..2592226 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T258854 WP_000244781.1 NZ_CP104649:c2588067-2587660 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT258854 WP_000354046.1 NZ_CP104649:c2588314-2588048 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References