Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2658632..2659129 Replicon chromosome
Accession NZ_CP104647
Organism Escherichia coli strain PNUSAE008406

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5921_RS13370 Protein ID WP_001173343.1
Coordinates 2658956..2659129 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5921_RS13365 Protein ID WP_000398619.1
Coordinates 2658632..2658928 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5921_RS13345 (2655363) 2655363..2656082 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5921_RS13350 (2656079) 2656079..2657710 - 1632 WP_001216477.1 sensor histidine kinase -
N5921_RS13355 (2657891) 2657891..2658121 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N5921_RS13360 (2658133) 2658133..2658405 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5921_RS13365 (2658632) 2658632..2658928 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5921_RS13370 (2658956) 2658956..2659129 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5921_RS13375 (2659248) 2659248..2660765 - 1518 WP_001295074.1 lysine--tRNA ligase -
N5921_RS13380 (2661002) 2661002..2662459 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258821 WP_001173343.1 NZ_CP104647:2658956-2659129 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258821 WP_000398619.1 NZ_CP104647:2658632-2658928 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References