Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2642755..2643252 Replicon chromosome
Accession NZ_CP104645
Organism Escherichia coli strain PNUSAE019785

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5932_RS13255 Protein ID WP_001173343.1
Coordinates 2643079..2643252 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5932_RS13250 Protein ID WP_000398619.1
Coordinates 2642755..2643051 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5932_RS13230 (2639486) 2639486..2640205 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5932_RS13235 (2640202) 2640202..2641833 - 1632 WP_001216477.1 sensor histidine kinase -
N5932_RS13240 (2642014) 2642014..2642244 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N5932_RS13245 (2642256) 2642256..2642528 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5932_RS13250 (2642755) 2642755..2643051 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5932_RS13255 (2643079) 2643079..2643252 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5932_RS13260 (2643371) 2643371..2644888 - 1518 WP_001295074.1 lysine--tRNA ligase -
N5932_RS13265 (2645125) 2645125..2646582 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258789 WP_001173343.1 NZ_CP104645:2643079-2643252 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258789 WP_000398619.1 NZ_CP104645:2642755-2643051 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References