Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1243467..1244121 Replicon chromosome
Accession NZ_CP104645
Organism Escherichia coli strain PNUSAE019785

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag N5932_RS06455 Protein ID WP_000244781.1
Coordinates 1243467..1243874 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N5932_RS06460 Protein ID WP_000354046.1
Coordinates 1243855..1244121 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5932_RS06435 (1239424) 1239424..1241157 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
N5932_RS06440 (1241163) 1241163..1241873 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N5932_RS06445 (1241898) 1241898..1242794 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
N5932_RS06450 (1242906) 1242906..1243427 + 522 WP_001055874.1 flavodoxin FldB -
N5932_RS06455 (1243467) 1243467..1243874 - 408 WP_000244781.1 protein YgfX Toxin
N5932_RS06460 (1243855) 1243855..1244121 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N5932_RS06465 (1244364) 1244364..1245344 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
N5932_RS06470 (1245421) 1245421..1246080 - 660 WP_000250274.1 hemolysin III family protein -
N5932_RS06475 (1246244) 1246244..1246555 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
N5932_RS06480 (1246600) 1246600..1248033 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T258781 WP_000244781.1 NZ_CP104645:c1243874-1243467 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT258781 WP_000354046.1 NZ_CP104645:c1244121-1243855 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References