Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2498409..2498906 Replicon chromosome
Accession NZ_CP104639
Organism Escherichia coli strain PNUSAE014970

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N4555_RS12145 Protein ID WP_001173343.1
Coordinates 2498733..2498906 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N4555_RS12140 Protein ID WP_000398619.1
Coordinates 2498409..2498705 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4555_RS12120 (2495140) 2495140..2495859 - 720 WP_000611288.1 two-component system response regulator DcuR -
N4555_RS12125 (2495856) 2495856..2497487 - 1632 WP_001216477.1 sensor histidine kinase -
N4555_RS12130 (2497668) 2497668..2497898 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N4555_RS12135 (2497910) 2497910..2498182 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N4555_RS12140 (2498409) 2498409..2498705 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N4555_RS12145 (2498733) 2498733..2498906 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N4555_RS12150 (2499025) 2499025..2500542 - 1518 WP_001295074.1 lysine--tRNA ligase -
N4555_RS12155 (2500779) 2500779..2502236 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258719 WP_001173343.1 NZ_CP104639:2498733-2498906 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258719 WP_000398619.1 NZ_CP104639:2498409-2498705 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References