Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1646993..1647793 Replicon chromosome
Accession NZ_CP104639
Organism Escherichia coli strain PNUSAE014970

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag N4555_RS08160 Protein ID WP_000342448.1
Coordinates 1646993..1647520 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag N4555_RS08165 Protein ID WP_001277108.1
Coordinates 1647527..1647793 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4555_RS08135 (1642068) 1642068..1642835 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
N4555_RS08140 (1642832) 1642832..1644109 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
N4555_RS08145 (1644106) 1644106..1645032 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
N4555_RS08150 (1645080) 1645080..1646189 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
N4555_RS08155 (1646613) 1646613..1646996 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
N4555_RS08160 (1646993) 1646993..1647520 - 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
N4555_RS08165 (1647527) 1647527..1647793 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
N4555_RS08170 (1647943) 1647943..1649046 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
N4555_RS08175 (1649317) 1649317..1650171 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
N4555_RS08180 (1650416) 1650416..1651474 - 1059 WP_001042018.1 permease-like cell division protein FtsX -
N4555_RS08185 (1651467) 1651467..1652135 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T258715 WP_000342448.1 NZ_CP104639:c1647520-1646993 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT258715 WP_001277108.1 NZ_CP104639:c1647793-1647527 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References