Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1108741..1109395 Replicon chromosome
Accession NZ_CP104639
Organism Escherichia coli strain PNUSAE014970

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag N4555_RS05425 Protein ID WP_000244781.1
Coordinates 1108741..1109148 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N4555_RS05430 Protein ID WP_000354046.1
Coordinates 1109129..1109395 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4555_RS05405 (1104698) 1104698..1106431 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
N4555_RS05410 (1106437) 1106437..1107147 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N4555_RS05415 (1107172) 1107172..1108068 - 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
N4555_RS05420 (1108180) 1108180..1108701 + 522 WP_001055874.1 flavodoxin FldB -
N4555_RS05425 (1108741) 1108741..1109148 - 408 WP_000244781.1 protein YgfX Toxin
N4555_RS05430 (1109129) 1109129..1109395 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N4555_RS05435 (1109638) 1109638..1110618 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
N4555_RS05440 (1110695) 1110695..1111354 - 660 WP_000250274.1 hemolysin III family protein -
N4555_RS05445 (1111518) 1111518..1111829 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
N4555_RS05450 (1111874) 1111874..1113307 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T258711 WP_000244781.1 NZ_CP104639:c1109148-1108741 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT258711 WP_000354046.1 NZ_CP104639:c1109395-1109129 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References