Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 173508..175131 Replicon plasmid pHNAH212872
Accession NZ_CP104630
Organism Escherichia coli strain AHM21C2872I

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag N5855_RS24315 Protein ID WP_000088046.1
Coordinates 173508..174833 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag N5855_RS24320 Protein ID WP_024136327.1
Coordinates 174838..175131 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5855_RS24290 (N5855_24240) 171238..171648 - 411 Protein_194 recombinase family protein -
N5855_RS24295 (N5855_24245) 171714..172418 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
N5855_RS24300 (N5855_24250) 172471..172716 - 246 Protein_196 HNH endonuclease -
N5855_RS24305 (N5855_24255) 172729..173139 - 411 WP_000985911.1 hypothetical protein -
N5855_RS24310 (N5855_24260) 173241..173447 - 207 WP_000134171.1 hypothetical protein -
N5855_RS24315 (N5855_24265) 173508..174833 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
N5855_RS24320 (N5855_24270) 174838..175131 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
N5855_RS24325 (N5855_24275) 175735..176127 - 393 WP_000341066.1 hypothetical protein -
N5855_RS24330 (N5855_24280) 176500..176841 + 342 WP_001151575.1 hypothetical protein -
N5855_RS24335 (N5855_24285) 176933..177124 + 192 WP_000398480.1 hypothetical protein -
N5855_RS24340 (N5855_24290) 177174..177455 + 282 WP_000077926.1 hypothetical protein -
N5855_RS24345 (N5855_24295) 177796..177927 - 132 WP_000374059.1 hypothetical protein -
N5855_RS24350 (N5855_24300) 178018..179259 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / lnu(F) / blaTEM-1B / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR - 1..273959 273959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T258688 WP_000088046.1 NZ_CP104630:c174833-173508 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT258688 WP_024136327.1 NZ_CP104630:c175131-174838 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References