Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2645202..2645785 Replicon chromosome
Accession NZ_CP104629
Organism Escherichia coli strain AHM21C2872I

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag N5855_RS12605 Protein ID WP_000254745.1
Coordinates 2645202..2645537 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1M2U551
Locus tag N5855_RS12610 Protein ID WP_021557150.1
Coordinates 2645537..2645785 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5855_RS12590 (2641089) 2641089..2642387 - 1299 WP_000036723.1 phosphopyruvate hydratase -
N5855_RS12595 (2642475) 2642475..2644112 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
N5855_RS12600 (2644340) 2644340..2645131 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
N5855_RS12605 (2645202) 2645202..2645537 - 336 WP_000254745.1 endoribonuclease MazF Toxin
N5855_RS12610 (2645537) 2645537..2645785 - 249 WP_021557150.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
N5855_RS12615 (2645863) 2645863..2648097 - 2235 WP_000226815.1 GTP pyrophosphokinase -
N5855_RS12620 (2648145) 2648145..2649446 - 1302 WP_021557151.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T258678 WP_000254745.1 NZ_CP104629:c2645537-2645202 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9383.79 Da        Isoelectric Point: 4.4665

>AT258678 WP_021557150.1 NZ_CP104629:c2645785-2645537 [Escherichia coli]
MIHSSVKRWGNSPAVRIPVTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2U551

References