Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 167646..169269 Replicon plasmid pHNAH212836K
Accession NZ_CP104628
Organism Klebsiella pneumoniae strain AHM21C2836KI

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag N5857_RS26135 Protein ID WP_000088046.1
Coordinates 167646..168971 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag N5857_RS26140 Protein ID WP_024136327.1
Coordinates 168976..169269 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5857_RS26110 (N5857_26105) 165376..165774 - 399 Protein_184 recombinase family protein -
N5857_RS26115 (N5857_26110) 165852..166556 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
N5857_RS26120 (N5857_26115) 166609..166854 - 246 Protein_186 HNH endonuclease -
N5857_RS26125 (N5857_26120) 166867..167277 - 411 WP_000985911.1 hypothetical protein -
N5857_RS26130 (N5857_26125) 167379..167585 - 207 WP_000134171.1 hypothetical protein -
N5857_RS26135 (N5857_26130) 167646..168971 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
N5857_RS26140 (N5857_26135) 168976..169269 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
N5857_RS26145 (N5857_26140) 169873..170265 - 393 WP_000341066.1 hypothetical protein -
N5857_RS26150 (N5857_26145) 170638..170979 + 342 WP_001151575.1 hypothetical protein -
N5857_RS26155 (N5857_26150) 171071..171262 + 192 WP_000398480.1 hypothetical protein -
N5857_RS26160 (N5857_26155) 171312..171593 + 282 WP_000077926.1 hypothetical protein -
N5857_RS26165 (N5857_26160) 171934..172065 - 132 WP_000374059.1 hypothetical protein -
N5857_RS26170 (N5857_26165) 172156..173397 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / lnu(F) / blaTEM-1B / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR - 1..266761 266761


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T258670 WP_000088046.1 NZ_CP104628:c168971-167646 [Klebsiella pneumoniae]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT258670 WP_024136327.1 NZ_CP104628:c169269-168976 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References