Detailed information of TA system TA025867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943075..943634
• Replicon: chromosome
• Accession: NC_016797
• Organism: Brucella suis VBI22

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BSVBI22_RS04535
• Protein ID: WP_004688321.1
• Coordinates: 943389..943634 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BSVBI22_RS04530
• Protein ID: WP_002964097.1
• Coordinates: 943075..943389 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSVBI22_RS04480	938765..938968	-	204	WP_004690812.1	hypothetical protein	-
BSVBI22_RS04485	938971..939186	-	216	WP_002971459.1	hypothetical protein	-
BSVBI22_RS04490	939183..939386	-	204	WP_002964088.1	hypothetical protein	-
BSVBI22_RS04495	939436..940155	+	720	WP_006072696.1	hypothetical protein	-
BSVBI22_RS04500	940152..940382	+	231	WP_002964091.1	hypothetical protein	-
BSVBI22_RS04505	940379..940654	+	276	WP_002964092.1	hypothetical protein	-
BSVBI22_RS04510	940677..941264	+	588	WP_004683738.1	hypothetical protein	-
BSVBI22_RS04515	941499..942209	+	711	WP_004683739.1	porin family protein	-
BSVBI22_RS04520	942557..942727	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BSVBI22_RS04530	943075..943389	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BSVBI22_RS04535	943389..943634	-	246	WP_004688321.1	BrnT family toxin	Toxin
BSVBI22_RS04540	943767..944405	+	639	Protein_890	tRNA-guanine transglycosylase	-
BSVBI22_RS17310	944475..945233	+	759	Protein_891	IS5 family transposase	-
BSVBI22_RS04550	945234..946007	+	774	WP_006132649.1	hypothetical protein	-
BSVBI22_RS04555	946142..947788	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	936993..951104	14111
	-	flank	IS/Tn	-	-	944475..944843	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T25867 WP_004688321.1 NC_016797:c943634-943389 [Brucella suis VBI22]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T25867 NC_016797:c943634-943389 [Brucella suis VBI22]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT25867 WP_002964097.1 NC_016797:c943389-943075 [Brucella suis VBI22]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT25867 NC_016797:c943389-943075 [Brucella suis VBI22]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA