Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4544970..4545780 Replicon chromosome
Accession NZ_CP104627
Organism Klebsiella pneumoniae strain AHM21C2836KI

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag N5857_RS22090 Protein ID WP_004178461.1
Coordinates 4544970..4545503 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag N5857_RS22095 Protein ID WP_002887278.1
Coordinates 4545514..4545780 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5857_RS22085 (4543801) 4543801..4544922 + 1122 WP_002887282.1 cupin domain-containing protein -
N5857_RS22090 (4544970) 4544970..4545503 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
N5857_RS22095 (4545514) 4545514..4545780 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
N5857_RS22100 (4545883) 4545883..4547316 - 1434 WP_117095269.1 Cu(+)/Ag(+) sensor histidine kinase -
N5857_RS22105 (4547306) 4547306..4547989 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
N5857_RS22110 (4548162) 4548162..4549547 + 1386 WP_080884167.1 efflux transporter outer membrane subunit -
N5857_RS22115 (4549565) 4549565..4549909 + 345 WP_065889379.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T258666 WP_004178461.1 NZ_CP104627:c4545503-4544970 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT258666 WP_002887278.1 NZ_CP104627:c4545780-4545514 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References