Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 91864..92662 Replicon plasmid pHNAH968
Accession NZ_CP104619
Organism Escherichia coli strain AHM9C68I

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag N5859_RS23075 Protein ID WP_032187665.1
Coordinates 92141..92662 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag N5859_RS23070 Protein ID WP_001711191.1
Coordinates 91864..92133 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5859_RS23045 (N5859_22955) 87291..88631 - 1341 WP_097296309.1 DnaB-like helicase C-terminal domain-containing protein -
N5859_RS23050 (N5859_22960) 88692..89417 - 726 WP_284632850.1 hypothetical protein -
N5859_RS23055 (N5859_22965) 89700..90467 + 768 WP_001711193.1 hypothetical protein -
N5859_RS23060 (N5859_22970) 90520..90873 - 354 WP_161953766.1 hypothetical protein -
N5859_RS23065 (N5859_22975) 90879..91544 - 666 WP_023135658.1 AAA family ATPase -
N5859_RS23070 (N5859_22980) 91864..92133 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
N5859_RS23075 (N5859_22985) 92141..92662 + 522 WP_032187665.1 GNAT family N-acetyltransferase Toxin
N5859_RS23080 (N5859_22990) 92831..93082 - 252 WP_023135660.1 hypothetical protein -
N5859_RS23085 (N5859_22995) 93260..93448 + 189 WP_000957857.1 hypothetical protein -
N5859_RS23090 (N5859_23000) 93458..94657 + 1200 WP_000948429.1 IS91 family transposase -
N5859_RS23095 (N5859_23005) 94821..95465 - 645 WP_284632852.1 hypothetical protein -
N5859_RS23100 (N5859_23010) 95479..95802 - 324 WP_001711185.1 hypothetical protein -
N5859_RS23105 (N5859_23015) 95936..96517 - 582 WP_213846801.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaNDM-1 / sul2 / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) / aph(3')-Ia htpB 1..133317 133317
- flank IS/Tn - - 93458..94657 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19345.11 Da        Isoelectric Point: 7.7881

>T258644 WP_032187665.1 NZ_CP104619:92141-92662 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT258644 WP_001711191.1 NZ_CP104619:91864-92133 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References