258623

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 34249..34892 Replicon plasmid pHNAH8212R
Accession NZ_CP104615
Organism Escherichia coli strain AHM8C212RI

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag N5856_RS24545 Protein ID WP_001044768.1
Coordinates 34476..34892 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag N5856_RS24540 Protein ID WP_001261287.1
Coordinates 34249..34479 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5856_RS24515 (N5856_24445) 30699..31721 + 1023 WP_000255944.1 IS21-like element IS100 family transposase -
N5856_RS24520 (N5856_24450) 31718..32500 + 783 WP_001300609.1 IS21-like element IS100kyp family helper ATPase IstB -
N5856_RS24525 (N5856_24455) 32539..32724 + 186 WP_284632464.1 hypothetical protein -
N5856_RS24530 (N5856_24460) 32774..33673 - 900 WP_000963206.1 nucleotide-binding protein -
N5856_RS24535 (N5856_24465) 33663..33953 - 291 WP_000111771.1 hypothetical protein -
N5856_RS24540 (N5856_24470) 34249..34479 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N5856_RS24545 (N5856_24475) 34476..34892 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
N5856_RS24550 (N5856_24480) 35054..37192 - 2139 WP_000350638.1 AAA family ATPase -
N5856_RS24555 (N5856_24485) 37546..37803 + 258 WP_000343085.1 hypothetical protein -
N5856_RS24560 (N5856_24490) 37803..38393 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / oqxA / oqxB / aph(3')-IIa / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B - 1..95335 95335


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T258623 WP_001044768.1 NZ_CP104615:34476-34892 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT258623 WP_001261287.1 NZ_CP104615:34249-34479 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References