Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 3392340..3393765 Replicon chromosome
Accession NZ_CP104613
Organism Escherichia coli strain AHM8C212RI

Toxin (Protein)


Gene name rnlA Uniprot ID A0A1X9TDS5
Locus tag N5856_RS16405 Protein ID WP_001730411.1
Coordinates 3392695..3393765 (-) Length 357 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID E6BSF0
Locus tag N5856_RS16400 Protein ID WP_000461705.1
Coordinates 3392340..3392702 (-) Length 121 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5856_RS16365 (3387472) 3387472..3389133 + 1662 WP_000880916.1 DNA repair protein RecN -
N5856_RS16370 (3389282) 3389282..3389623 + 342 WP_001203437.1 outer membrane protein assembly factor BamE -
N5856_RS16375 (3389685) 3389685..3389975 - 291 WP_001117838.1 RnfH family protein -
N5856_RS16380 (3389965) 3389965..3390402 - 438 WP_001341638.1 type II toxin-antitoxin system toxin RatA -
N5856_RS16385 (3390573) 3390573..3391055 + 483 WP_000162574.1 SsrA-binding protein SmpB -
N5856_RS16395 (3391933) 3391933..3392202 + 270 WP_000429347.1 hypothetical protein -
N5856_RS16400 (3392340) 3392340..3392702 - 363 WP_000461705.1 type II toxin-antitoxin system RnlB family antitoxin Antitoxin
N5856_RS16405 (3392695) 3392695..3393765 - 1071 WP_001730411.1 type II toxin-antitoxin system RnlA family toxin Toxin
N5856_RS16410 (3393900) 3393900..3394096 - 197 Protein_3207 recombinase family protein -
N5856_RS16420 (3395427) 3395427..3396737 + 1311 Protein_3209 alpha amylase C-terminal domain-containing protein -
N5856_RS16425 (3397075) 3397075..3398052 + 978 WP_032222549.1 glutarate dioxygenase GlaH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(15-112)


Sequences

Toxin        


Download         Length: 357 a.a.        Molecular weight: 40463.36 Da        Isoelectric Point: 6.8748

>T258611 WP_001730411.1 NZ_CP104613:c3393765-3392695 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANHNL
GKELADDLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGRYFDKIGSEFILKQQFGCNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Download         Length: 1071 bp

Antitoxin


Download         Length: 121 a.a.        Molecular weight: 13304.23 Da        Isoelectric Point: 4.2386

>AT258611 WP_000461705.1 NZ_CP104613:c3392702-3392340 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Download         Length: 363 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X9TDS5

Antitoxin

Source ID Structure
AlphaFold DB A0A1X9TDS3

References