Detailed information of TA system TA025860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 480678..481315
• Accession: NC_016784
• Organism: Bacillus amyloliquefaciens subsp. plantarum CAU B946

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BACAU_RS02475
• Protein ID: WP_003156187.1
• Coordinates: 480965..481315 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BACAU_RS02470
• Protein ID: WP_003156188.1
• Coordinates: 480678..480959 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BACAU_RS02450	477043..477642	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
BACAU_RS02455	477735..478100	+	366	WP_014304402.1	holo-ACP synthase	-
BACAU_RS02460	478265..479272	+	1008	WP_014304403.1	outer membrane lipoprotein carrier protein LolA	-
BACAU_RS02465	479389..480558	+	1170	WP_003156189.1	alanine racemase	-
BACAU_RS02470	480678..480959	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BACAU_RS02475	480965..481315	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BACAU_RS02480	481433..482254	+	822	WP_014304404.1	STAS domain-containing protein	-
BACAU_RS02485	482259..482624	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BACAU_RS02490	482627..483028	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BACAU_RS02495	483040..484047	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
BACAU_RS02500	484111..484440	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BACAU_RS02505	484437..484919	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
BACAU_RS02510	484885..485673	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BACAU_RS02515	485673..486275	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T25860 WP_003156187.1 NC_016784:480965-481315 [Bacillus velezensis CAU B946]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T25860 NC_016784:480965-481315 [Bacillus velezensis CAU B946]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT25860 WP_003156188.1 NC_016784:480678-480959 [Bacillus velezensis CAU B946]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT25860 NC_016784:480678-480959 [Bacillus velezensis CAU B946]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5