Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2729..3321 Replicon plasmid pHNAH8161B-5
Accession NZ_CP104609
Organism Klebsiella pneumoniae strain AHM8C161BI

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag N5862_RS30730 Protein ID WP_000520549.1
Coordinates 2729..2977 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag N5862_RS30735 Protein ID WP_000681613.1
Coordinates 2974..3321 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5862_RS30705 (N5862_30695) 226..768 + 543 WP_001083906.1 hypothetical protein -
N5862_RS30710 (N5862_30700) 765..1016 + 252 WP_001230707.1 hypothetical protein -
N5862_RS30715 (N5862_30705) 1003..1230 + 228 WP_000801440.1 hypothetical protein -
N5862_RS30720 (N5862_30710) 1684..1983 + 300 WP_000650309.1 hypothetical protein -
N5862_RS30725 (N5862_30715) 2074..2424 + 351 WP_000854259.1 hypothetical protein -
N5862_RS30730 (N5862_30720) 2729..2977 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
N5862_RS30735 (N5862_30725) 2974..3321 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N5862_RS30740 (N5862_30730) 3682..4185 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
N5862_RS30745 (N5862_30735) 4189..5418 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
N5862_RS30750 (N5862_30740) 5495..5788 + 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
N5862_RS30755 (N5862_30745) 5785..6000 + 216 WP_000760375.1 EexN family lipoprotein -
N5862_RS30760 (N5862_30750) 6356..6976 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
N5862_RS30765 (N5862_30755) 6990..7316 + 327 WP_000008647.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33310 33310


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T258596 WP_000520549.1 NZ_CP104609:2729-2977 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT258596 WP_000681613.1 NZ_CP104609:2974-3321 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References