Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 250259..250901 Replicon chromosome
Accession NC_016779
Organism Bacillus cereus F837/76

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BCF_RS01435 Protein ID WP_000635963.1
Coordinates 250551..250901 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCF_RS01430 Protein ID WP_000004570.1
Coordinates 250259..250546 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCF_RS01405 245575..246537 + 963 WP_000961158.1 UV DNA damage repair endonuclease UvsE -
BCF_RS01410 246530..247102 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BCF_RS01415 247195..247554 + 360 WP_000635040.1 holo-ACP synthase -
BCF_RS01420 247711..248661 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BCF_RS01425 248780..249949 + 1170 WP_000390600.1 alanine racemase -
BCF_RS01430 250259..250546 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCF_RS01435 250551..250901 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCF_RS01440 250969..253137 + 2169 WP_000426222.1 RNA-binding transcriptional accessory protein -
BCF_RS01445 253195..253311 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCF_RS01450 253507..253965 + 459 WP_000344250.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T25855 WP_000635963.1 NC_016779:250551-250901 [Bacillus cereus F837/76]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T25855 NC_016779:250551-250901 [Bacillus cereus F837/76]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT25855 WP_000004570.1 NC_016779:250259-250546 [Bacillus cereus F837/76]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT25855 NC_016779:250259-250546 [Bacillus cereus F837/76]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References