Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 274778..275275 Replicon chromosome
Accession NZ_CP104598
Organism Escherichia coli strain AHM8C160RI

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5861_RS01275 Protein ID WP_001173343.1
Coordinates 275102..275275 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5861_RS01270 Protein ID WP_000398619.1
Coordinates 274778..275074 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5861_RS01250 (271509) 271509..272228 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5861_RS01255 (272225) 272225..273856 - 1632 WP_094317780.1 sensor histidine kinase -
N5861_RS01260 (274037) 274037..274267 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
N5861_RS01265 (274279) 274279..274551 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5861_RS01270 (274778) 274778..275074 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5861_RS01275 (275102) 275102..275275 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5861_RS01280 (275394) 275394..276911 - 1518 WP_001295074.1 lysine--tRNA ligase -
N5861_RS01285 (277148) 277148..278605 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258545 WP_001173343.1 NZ_CP104598:275102-275275 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258545 WP_000398619.1 NZ_CP104598:274778-275074 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References