Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 34446..35089 Replicon plasmid pEC9952-1
Accession NZ_CP104596
Organism Escherichia coli strain EC9952

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag N5B57_RS24025 Protein ID WP_001034044.1
Coordinates 34673..35089 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag N5B57_RS24020 Protein ID WP_001261286.1
Coordinates 34446..34676 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5B57_RS24005 (29583) 29583..29813 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
N5B57_RS24010 (29810) 29810..30226 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
N5B57_RS24015 (30271) 30271..34065 - 3795 WP_001144732.1 hypothetical protein -
N5B57_RS24020 (34446) 34446..34676 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N5B57_RS24025 (34673) 34673..35089 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
N5B57_RS24030 (35164) 35164..36729 + 1566 WP_001128474.1 AAA family ATPase -
N5B57_RS24035 (36714) 36714..37736 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / aph(3')-Ia / sul3 / blaCTX-M-27 / mph(A) / aac(3)-IId / blaTEM-1B iutA / iucD / iucC / iucB / iucA 1..157264 157264
- flank IS/Tn - - 37990..38493 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T258544 WP_001034044.1 NZ_CP104596:34673-35089 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT258544 WP_001261286.1 NZ_CP104596:34446-34676 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References