Detailed information of TA system TA025854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 111212..111771
• Replicon: chromosome
• Accession: NC_016778
• Organism: Brucella canis HSK A52141

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BCA52141_RS00495
• Protein ID: WP_004688321.1
• Coordinates: 111212..111457 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BCA52141_RS00500
• Protein ID: WP_002964097.1
• Coordinates: 111457..111771 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCA52141_RS00470	107059..108705	-	1647	WP_004689673.1	hypothetical protein	-
BCA52141_RS00475	108840..109613	-	774	WP_006132649.1	hypothetical protein	-
BCA52141_RS00490	110441..111079	-	639	Protein_95	tRNA-guanine transglycosylase	-
BCA52141_RS00495	111212..111457	+	246	WP_004688321.1	BrnT family toxin	Toxin
BCA52141_RS00500	111457..111771	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BCA52141_RS00505	112119..112289	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
BCA52141_RS00510	112637..113347	-	711	Protein_99	porin family protein	-
BCA52141_RS00515	113582..114169	-	588	WP_004683738.1	hypothetical protein	-
BCA52141_RS00520	114192..114467	-	276	WP_002964092.1	hypothetical protein	-
BCA52141_RS00525	114464..114694	-	231	WP_002964091.1	hypothetical protein	-
BCA52141_RS00530	114691..115410	-	720	WP_006072696.1	hypothetical protein	-
BCA52141_RS00535	115460..115663	+	204	WP_002964088.1	hypothetical protein	-
BCA52141_RS00540	115660..115875	+	216	WP_002971459.1	hypothetical protein	-
BCA52141_RS00545	115878..116081	+	204	WP_004690812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	103945..117853	13908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T25854 WP_004688321.1 NC_016778:111212-111457 [Brucella canis HSK A52141]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T25854 NC_016778:111212-111457 [Brucella canis HSK A52141]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT25854 WP_002964097.1 NC_016778:111457-111771 [Brucella canis HSK A52141]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT25854 NC_016778:111457-111771 [Brucella canis HSK A52141]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA