Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4444708..4445205 Replicon chromosome
Accession NZ_CP104595
Organism Escherichia coli strain EC9952

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5B57_RS21585 Protein ID WP_001173343.1
Coordinates 4444708..4444881 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5B57_RS21590 Protein ID WP_000398619.1
Coordinates 4444909..4445205 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5B57_RS21575 (4441378) 4441378..4442835 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
N5B57_RS21580 (4443072) 4443072..4444589 + 1518 WP_001295074.1 lysine--tRNA ligase -
N5B57_RS21585 (4444708) 4444708..4444881 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5B57_RS21590 (4444909) 4444909..4445205 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5B57_RS21595 (4445432) 4445432..4445704 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5B57_RS21600 (4445716) 4445716..4445946 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
N5B57_RS21605 (4446127) 4446127..4447758 + 1632 WP_001216486.1 sensor histidine kinase -
N5B57_RS21610 (4447755) 4447755..4448474 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258537 WP_001173343.1 NZ_CP104595:c4444881-4444708 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258537 WP_000398619.1 NZ_CP104595:c4445205-4444909 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References