Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 276649..277235 Replicon chromosome
Accession NZ_CP104595
Organism Escherichia coli strain EC9952

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag N5B57_RS01240 Protein ID WP_042014162.1
Coordinates 276649..276879 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag N5B57_RS01245 Protein ID WP_000593555.1
Coordinates 276876..277235 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5B57_RS01230 (272686) 272686..275421 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
N5B57_RS01235 (275421) 275421..276545 + 1125 WP_001314210.1 ABC transporter permease -
N5B57_RS01240 (276649) 276649..276879 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
N5B57_RS01245 (276876) 276876..277235 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N5B57_RS01250 (277355) 277355..277756 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
N5B57_RS01255 (277762) 277762..278568 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
N5B57_RS01260 (278565) 278565..279329 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
N5B57_RS01265 (279329) 279329..280162 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
N5B57_RS01270 (280159) 280159..281103 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T258520 WP_042014162.1 NZ_CP104595:276649-276879 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT258520 WP_000593555.1 NZ_CP104595:276876-277235 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References