258519

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 418928..419621 Replicon plasmid pWTJH36
Accession NZ_CP104591
Organism Pseudomonas aeruginosa strain WTJH36

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag N5878_RS35675 Protein ID WP_003151133.1
Coordinates 418928..419305 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag N5878_RS35680 Protein ID WP_001172026.1
Coordinates 419286..419621 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5878_RS35660 (N5878_35660) 414583..415164 + 582 WP_260890468.1 hypothetical protein -
N5878_RS35665 (N5878_35665) 415121..418150 - 3030 WP_010799689.1 Tn3 family transposase -
N5878_RS35670 (N5878_35670) 418134..418736 - 603 WP_010465829.1 recombinase family protein -
N5878_RS35675 (N5878_35675) 418928..419305 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5878_RS35680 (N5878_35680) 419286..419621 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
N5878_RS35685 (N5878_35685) 419636..419971 + 336 WP_000741275.1 hypothetical protein -
N5878_RS35690 (N5878_35690) 419995..420321 + 327 WP_000091614.1 hypothetical protein -
N5878_RS35695 (N5878_35695) 420318..420689 + 372 WP_172691856.1 hypothetical protein -
N5878_RS35700 (N5878_35700) 420784..421206 + 423 WP_260890453.1 hypothetical protein -
N5878_RS35705 (N5878_35705) 421234..421941 - 708 WP_034036499.1 hypothetical protein -
N5878_RS35710 (N5878_35710) 422112..422213 + 102 Protein_485 prepilin-type N-terminal cleavage/methylation domain-containing protein -
N5878_RS35715 (N5878_35715) 423124..423948 + 825 WP_038405176.1 Ig-like domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaVIM-24 / blaOXA-101 / ant(3'')-Ia / qacE / sul1 / aph(3'')-Ib / aph(6)-Id - 1..462066 462066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T258519 WP_003151133.1 NZ_CP104591:418928-419305 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT258519 WP_001172026.1 NZ_CP104591:419286-419621 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References