Detailed information of TA system TA025851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3763368..3763897
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: TBSG_RS17715
• Protein ID: WP_003417760.1
• Coordinates: 3763640..3763897 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: TBSG_RS17710
• Protein ID: WP_003417757.1
• Coordinates: 3763368..3763643 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS22465	3760166..3761603	-	1438	Protein_3455	FAD-binding oxidoreductase	-
TBSG_RS17695	3761706..3762095	+	390	WP_003417745.1	DUF732 domain-containing protein	-
TBSG_RS17700	3762109..3762402	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
TBSG_RS17705	3762399..3763244	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
TBSG_RS17710	3763368..3763643	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
TBSG_RS17715	3763640..3763897	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
TBSG_RS17720	3763939..3765129	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
TBSG_RS17725	3765246..3765614	+	369	WP_003417765.1	FHA domain-containing protein	-
TBSG_RS17730	3765611..3766162	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
TBSG_RS17735	3766169..3766750	-	582	WP_003417769.1	ATP/GTP-binding protein	-
TBSG_RS17740	3766731..3767099	-	369	WP_003417772.1	DUF742 domain-containing protein	-
TBSG_RS17745	3767077..3767469	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T25851 WP_003417760.1 NC_016768:3763640-3763897 [Mycobacterium tuberculosis KZN 4207]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T25851 NC_016768:3763640-3763897 [Mycobacterium tuberculosis KZN 4207]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT25851 WP_003417757.1 NC_016768:3763368-3763643 [Mycobacterium tuberculosis KZN 4207]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT25851 NC_016768:3763368-3763643 [Mycobacterium tuberculosis KZN 4207]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94