Detailed information of TA system TA025850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3702433..3703107
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: TBSG_RS17525
• Protein ID: WP_003417282.1
• Coordinates: 3702433..3702861 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: TBSG_RS17530
• Protein ID: WP_003417286.1
• Coordinates: 3702865..3703107 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS17500	3698255..3698656	-	402	WP_003417264.1	cytidine deaminase	-
TBSG_RS17505	3698797..3699231	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
TBSG_RS17510	3699228..3699662	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
TBSG_RS17515	3699791..3701563	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
TBSG_RS17520	3701563..3702354	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
TBSG_RS17525	3702433..3702861	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBSG_RS17530	3702865..3703107	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
TBSG_RS17535	3703229..3703843	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
TBSG_RS17540	3703840..3704505	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
TBSG_RS17545	3704506..3705039	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
TBSG_RS17550	3705036..3705410	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
TBSG_RS17565	3706784..3706975	-	192	Protein_3432	RNA polymerase sigma factor SigJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T25850 WP_003417282.1 NC_016768:c3702861-3702433 [Mycobacterium tuberculosis KZN 4207]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T25850 NC_016768:c3702861-3702433 [Mycobacterium tuberculosis KZN 4207]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT25850 WP_003417286.1 NC_016768:c3703107-3702865 [Mycobacterium tuberculosis KZN 4207]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT25850 NC_016768:c3703107-3702865 [Mycobacterium tuberculosis KZN 4207]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59