Detailed information of TA system TA025848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3393410..3394029
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: TBSG_RS16045
• Protein ID: WP_003404726.1
• Coordinates: 3393410..3393844 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: TBSG_RS16050
• Protein ID: WP_003898641.1
• Coordinates: 3393850..3394029 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS16020	3388745..3389983	+	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-
TBSG_RS16025	3389985..3391493	+	1509	WP_003404742.1	carotenoid oxygenase family protein	-
TBSG_RS16030	3391660..3391890	+	231	WP_003898642.1	hypothetical protein	-
TBSG_RS16035	3392025..3392474	-	450	WP_003404738.1	hypothetical protein	-
TBSG_RS16040	3392539..3393312	-	774	WP_003404735.1	VOC family protein	-
TBSG_RS16045	3393410..3393844	-	435	WP_003404726.1	SRPBCC family protein	Toxin
TBSG_RS16050	3393850..3394029	-	180	WP_003898641.1	antitoxin	Antitoxin
TBSG_RS22440	3394155..3394313	-	159	WP_003404720.1	hypothetical protein	-
TBSG_RS16055	3394586..3396979	-	2394	WP_003898639.1	cation-translocating P-type ATPase	-
TBSG_RS16060	3396976..3398556	-	1581	WP_003909313.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T25848 WP_003404726.1 NC_016768:c3393844-3393410 [Mycobacterium tuberculosis KZN 4207]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T25848 NC_016768:c3393844-3393410 [Mycobacterium tuberculosis KZN 4207]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT25848 WP_003898641.1 NC_016768:c3394029-3393850 [Mycobacterium tuberculosis KZN 4207]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT25848 NC_016768:c3394029-3393850 [Mycobacterium tuberculosis KZN 4207]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5