Detailed information of TA system TA258472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1283059..1284461
• Replicon: chromosome
• Accession: NZ_CP104582
• Organism: Pseudomonas sp. GD03919

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: N5O87_RS06215
• Protein ID: WP_279532435.1
• Coordinates: 1283059..1283628 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: N5O87_RS06220
• Protein ID: WP_279532436.1
• Coordinates: 1283634..1284461 (+)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N5O87_RS06210 (N5O87_06210)	1279376..1282864	-	3489	WP_147809655.1	hybrid sensor histidine kinase/response regulator	-
N5O87_RS06215 (N5O87_06215)	1283059..1283628	+	570	WP_279532435.1	GNAT family protein	Toxin
N5O87_RS06220 (N5O87_06220)	1283634..1284461	+	828	WP_279532436.1	AraC family transcriptional regulator	Antitoxin
N5O87_RS06225 (N5O87_06225)	1285350..1286687	-	1338	WP_279532437.1	imelysin family protein	-
N5O87_RS06230 (N5O87_06230)	1286878..1287450	+	573	WP_279532438.1	LemA family protein	-
N5O87_RS06235 (N5O87_06235)	1287461..1288366	+	906	WP_279532439.1	GIDE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB	1154761..1354897	200136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• DNA binding HTH domain, AraC-type (193-270)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21394.61 Da
• Isoelectric Point: 8.1211

>T258472 WP_279532435.1 NZ_CP104582:1283059-1283628 [Pseudomonas sp. GD03919]
MFKPQLVTLQRGALRLEPMADADIPALVALAEANREALLYMSGTQRLDWYRSALADLREQRALPFVIRLGDELVGTTRFA
DFMNSLPACEIGWTWLDRHQHGSGLNTSIKYLMLRHAFDHWGMVRVQLKTAASNLRAQRAIEKLGAVREGLLRNHRRLAD
GRLDDSVLFSITDHEWPALREAMEAGFGA

Nucleotide

• Length: 570 bp

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30799.20 Da
• Isoelectric Point: 8.7287

>AT258472 WP_279532436.1 NZ_CP104582:1283634-1284461 [Pseudomonas sp. GD03919]
MQTREQTRFWQASALSGVELLQARYLEQRFAPHVHEGFVFTVIEHGAQRFRHRGCDHLAPQGSMVLINPDEVHTGSKAHE
DGWRYRGFYPDNAQVLGALQELGLAKAGMPSFTFSVLHDPRLHAAFLQLHQLLEGGAEALQQQLAWREAILLLFQHHAQL
AEPPAPGREPWAVACAKQMLAERLIEPPSLEELAAAVNLSPFHFARVFRRATGLPPHAWLKQRRLEQARALLKTGCTAVA
VAMQLGFADQSHLSRQFKQVYGVSPGEYRSAVARS

Nucleotide

• Length: 828 bp