258451

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1144200..1145791 Replicon chromosome
Accession NZ_CP104579
Organism Pseudomonas oleovorans strain GD04132

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag N5O83_RS05485 Protein ID WP_279534532.1
Coordinates 1144200..1145525 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID W6RJ44
Locus tag N5O83_RS05490 Protein ID WP_003459718.1
Coordinates 1145525..1145791 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O83_RS05455 (N5O83_05460) 1139765..1140547 + 783 WP_003459710.1 amidohydrolase -
N5O83_RS05460 (N5O83_05465) 1140563..1141018 + 456 WP_003459711.1 hypothetical protein -
N5O83_RS05465 (N5O83_05470) 1141307..1141510 + 204 WP_279534491.1 IS66 family insertion sequence element accessory protein TnpB -
N5O83_RS05470 (N5O83_05475) 1142022..1143290 + 1269 WP_003459714.1 group II intron reverse transcriptase/maturase -
N5O83_RS05475 (N5O83_05480) 1143324..1143527 + 204 WP_256672073.1 transposase -
N5O83_RS05480 (N5O83_05485) 1143547..1143927 + 381 Protein_1083 transposase -
N5O83_RS05485 (N5O83_05490) 1144200..1145525 + 1326 WP_279534532.1 HipA domain-containing protein Toxin
N5O83_RS05490 (N5O83_05495) 1145525..1145791 + 267 WP_003459718.1 helix-turn-helix transcriptional regulator Antitoxin
N5O83_RS05495 (N5O83_05500) 1147053..1147484 + 432 WP_003460108.1 IS200/IS605 family transposase -
N5O83_RS05500 (N5O83_05505) 1147498..1147869 - 372 WP_279534533.1 hypothetical protein -
N5O83_RS05505 (N5O83_05510) 1147915..1149414 + 1500 WP_279534921.1 IS21 family transposase -
N5O83_RS05510 (N5O83_05515) 1149407..1150210 + 804 WP_003459721.1 IS21-like element ISPst3 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1142022..1151555 9533
- inside IScluster/Tn - - 1141307..1150210 8903


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-385)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 49108.64 Da        Isoelectric Point: 4.8209

>T258451 WP_279534532.1 NZ_CP104579:1144200-1145525 [Pseudomonas oleovorans]
MHNLTLQVYRDGQWRNAVELRFEQPEEGLEGACAYGYQQGYLLDALESVGTRLNVAVSASYPLGWDSWRDKKAPAFLFDI
LPSGAARRFLLKRLGGERPEGLNLDLYLLGRCTPAPIGNLRIKESVEAIAGSPVLGFTRDEVISRDSRFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDRQGALHPDAVLPDADAAQHWFVKFARNKASQTDQDILRSEYCFYRAVQQLGMDTVAAEGLAL
EEGNKPSLWMHRFDREISSTGVCRTAVESMYSLCGVTEAGSYMLHTQVLGKLVELWRAVGQEQQVGDLVFEYMRRDLLNQ
ILGNSDNHGRNTAILRSEQGVRLAPIYDLAPMVMDEEGVTRTTKWPKHIELAGDVNWRAACDEVAEWIDADELFQRLQLA
AQDLLALPDLLSAQGLPDSTMSHPRIALRNLQQRMLQWGLV

Download         Length: 1326 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9933.55 Da        Isoelectric Point: 10.9502

>AT258451 WP_003459718.1 NZ_CP104579:1145525-1145791 [Pseudomonas oleovorans]
MDILERNRILNELQQQLASGEITIGGAVRRLRKEVTGLQQARFAQMCKLSLRALRQLELDESNPTVQTLNSVFNPFGMQV
GIVPKTPR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB W6RJ44

References