25845

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3177619..3178250 Replicon chromosome
Accession NC_016768
Organism Mycobacterium tuberculosis KZN 4207

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag TBSG_RS14980 Protein ID WP_003898728.1
Coordinates 3177939..3178250 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag TBSG_RS14975 Protein ID WP_003405836.1
Coordinates 3177619..3177939 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBSG_RS14945 3172815..3173453 + 639 WP_003898730.1 lipid droplet-associated protein -
TBSG_RS14950 3173450..3174697 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
TBSG_RS14955 3174687..3174944 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
TBSG_RS14960 3174954..3176066 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
TBSG_RS21530 3176084..3177609 - 1526 Protein_2929 carboxylesterase/lipase family protein -
TBSG_RS14975 3177619..3177939 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
TBSG_RS14980 3177939..3178250 + 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
TBSG_RS14985 3178362..3179519 + 1158 WP_003898727.1 AI-2E family transporter -
TBSG_RS14990 3179526..3180170 - 645 WP_003915494.1 DUF4245 domain-containing protein -
TBSG_RS14995 3180226..3181314 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
TBSG_RS15000 3181345..3182769 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T25845 WP_003898728.1 NC_016768:3177939-3178250 [Mycobacterium tuberculosis KZN 4207]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T25845 NC_016768:3177939-3178250 [Mycobacterium tuberculosis KZN 4207]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT25845 WP_003405836.1 NC_016768:3177619-3177939 [Mycobacterium tuberculosis KZN 4207]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT25845 NC_016768:3177619-3177939 [Mycobacterium tuberculosis KZN 4207]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References