Detailed information of TA system TA025844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3168926..3169494
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: TBSG_RS14920
• Protein ID: WP_003405865.1
• Coordinates: 3168926..3169300 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: TBSG_RS14925
• Protein ID: WP_003405863.1
• Coordinates: 3169297..3169494 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS14885	3165339..3166046	+	708	Protein_2913	adenylate/guanylate cyclase domain-containing protein	-
TBSG_RS22400	3165998..3166207	-	210	WP_003911400.1	hypothetical protein	-
TBSG_RS14895	3166229..3166939	+	711	WP_003911399.1	hypothetical protein	-
TBSG_RS14900	3166954..3167277	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
TBSG_RS14905	3167520..3167795	+	276	WP_003405867.1	hypothetical protein	-
TBSG_RS14910	3167722..3167907	-	186	WP_003901093.1	hypothetical protein	-
TBSG_RS14915	3168025..3168723	-	699	WP_003898733.1	hypothetical protein	-
TBSG_RS14920	3168926..3169300	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBSG_RS14925	3169297..3169494	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBSG_RS14930	3169582..3170655	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
TBSG_RS14935	3170718..3171701	+	984	WP_003898731.1	hypothetical protein	-
TBSG_RS14940	3171718..3172725	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
TBSG_RS14945	3172815..3173453	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T25844 WP_003405865.1 NC_016768:c3169300-3168926 [Mycobacterium tuberculosis KZN 4207]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T25844 NC_016768:c3169300-3168926 [Mycobacterium tuberculosis KZN 4207]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT25844 WP_003405863.1 NC_016768:c3169494-3169297 [Mycobacterium tuberculosis KZN 4207]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT25844 NC_016768:c3169494-3169297 [Mycobacterium tuberculosis KZN 4207]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07