Detailed information of TA system TA025843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3023998..3024686
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: TBSG_RS14255
• Protein ID: WP_003406304.1
• Coordinates: 3023998..3024429 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: TBSG_RS14260
• Protein ID: WP_003406302.1
• Coordinates: 3024426..3024686 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS14230	3019720..3019989	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TBSG_RS14235	3019986..3020279	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
TBSG_RS14240	3020336..3021166	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
TBSG_RS14245	3021247..3022107	-	861	WP_003900301.1	hypothetical protein	-
TBSG_RS14250	3022287..3023975	+	1689	WP_003910308.1	PE family protein	-
TBSG_RS14255	3023998..3024429	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBSG_RS14260	3024426..3024686	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
TBSG_RS14265	3024762..3025751	-	990	WP_003406301.1	malate dehydrogenase	-
TBSG_RS14270	3025922..3027022	+	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
TBSG_RS14275	3027099..3028280	-	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
TBSG_RS14280	3028285..3029109	-	825	WP_003900298.1	trehalose ABC transporter permease SugB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T25843 WP_003406304.1 NC_016768:c3024429-3023998 [Mycobacterium tuberculosis KZN 4207]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T25843 NC_016768:c3024429-3023998 [Mycobacterium tuberculosis KZN 4207]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT25843 WP_003406302.1 NC_016768:c3024686-3024426 [Mycobacterium tuberculosis KZN 4207]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT25843 NC_016768:c3024686-3024426 [Mycobacterium tuberculosis KZN 4207]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6