Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4730801..4731611 Replicon chromosome
Accession NZ_CP104551
Organism Klebsiella pneumoniae strain K28074

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag N5911_RS23510 Protein ID WP_002887280.1
Coordinates 4730801..4731334 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag N5911_RS23515 Protein ID WP_002887278.1
Coordinates 4731345..4731611 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5911_RS23505 (N5911_23505) 4729632..4730753 + 1122 WP_002887282.1 cupin domain-containing protein -
N5911_RS23510 (N5911_23510) 4730801..4731334 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
N5911_RS23515 (N5911_23515) 4731345..4731611 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
N5911_RS23520 (N5911_23520) 4731714..4733147 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
N5911_RS23525 (N5911_23525) 4733137..4733820 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
N5911_RS23530 (N5911_23530) 4733992..4735377 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
N5911_RS23535 (N5911_23535) 4735395..4735739 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T258420 WP_002887280.1 NZ_CP104551:c4731334-4730801 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT258420 WP_002887278.1 NZ_CP104551:c4731611-4731345 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References