Detailed information of TA system TA025842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3019720..3020279
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: TBSG_RS14235
• Protein ID: WP_003898789.1
• Coordinates: 3019986..3020279 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: TBSG_RS14230
• Protein ID: WP_003406322.1
• Coordinates: 3019720..3019989 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS14220	3014982..3015770	+	789	WP_003406325.1	hypothetical protein	-
TBSG_RS14225	3015912..3019607	+	3696	WP_009938349.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
TBSG_RS14230	3019720..3019989	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TBSG_RS14235	3019986..3020279	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
TBSG_RS14240	3020336..3021166	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
TBSG_RS14245	3021247..3022107	-	861	WP_003900301.1	hypothetical protein	-
TBSG_RS14250	3022287..3023975	+	1689	WP_003910308.1	PE family protein	-
TBSG_RS14255	3023998..3024429	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
TBSG_RS14260	3024426..3024686	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T25842 WP_003898789.1 NC_016768:3019986-3020279 [Mycobacterium tuberculosis KZN 4207]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T25842 NC_016768:3019986-3020279 [Mycobacterium tuberculosis KZN 4207]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT25842 WP_003406322.1 NC_016768:3019720-3019989 [Mycobacterium tuberculosis KZN 4207]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT25842 NC_016768:3019720-3019989 [Mycobacterium tuberculosis KZN 4207]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2