258400

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 35454..35979 Replicon plasmid p2
Accession NZ_CP104541
Organism Escherichia coli strain E6

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag N5O76_RS24820 Protein ID WP_001159871.1
Coordinates 35454..35759 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag N5O76_RS24825 Protein ID WP_000813639.1
Coordinates 35761..35979 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O76_RS24790 (31450) 31450..31623 + 174 Protein_33 DUF4113 domain-containing protein -
N5O76_RS24795 (31625) 31625..32041 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
N5O76_RS24800 (32034) 32034..33014 - 981 WP_000688514.1 plasmid segregation protein ParM -
N5O76_RS24805 (33428) 33428..33736 - 309 WP_000030199.1 hypothetical protein -
N5O76_RS24810 (33823) 33823..34467 - 645 WP_001144036.1 ParA family protein -
N5O76_RS24815 (34647) 34647..35453 - 807 WP_033904598.1 site-specific integrase -
N5O76_RS24820 (35454) 35454..35759 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
N5O76_RS24825 (35761) 35761..35979 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
N5O76_RS24830 (36576) 36576..36836 + 261 WP_000343102.1 hypothetical protein -
N5O76_RS24835 (36833) 36833..37423 + 591 WP_000194555.1 hypothetical protein -
N5O76_RS24840 (37441) 37441..37788 - 348 WP_000142424.1 DUF6404 family protein -
N5O76_RS24845 (37907) 37907..38254 + 348 WP_000762580.1 colicin E1 family microcin immunity protein -
N5O76_RS24850 (38272) 38272..39897 - 1626 WP_001456214.1 colicin Ia central receptor-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aac(3)-VIa / ant(3'')-Ia - 1..114993 114993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T258400 WP_001159871.1 NZ_CP104541:c35759-35454 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT258400 WP_000813639.1 NZ_CP104541:c35979-35761 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References