258389

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 83636..84279 Replicon plasmid p1
Accession NZ_CP104540
Organism Escherichia coli strain E6

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag N5O76_RS24065 Protein ID WP_001034044.1
Coordinates 83863..84279 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag N5O76_RS24060 Protein ID WP_001261286.1
Coordinates 83636..83866 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O76_RS24035 (79144) 79144..79971 + 828 WP_000154003.1 HAD-IIB family hydrolase -
N5O76_RS24040 (79974) 79974..80990 + 1017 WP_000583428.1 Gfo/Idh/MocA family oxidoreductase -
N5O76_RS24045 (80992) 80992..81261 + 270 WP_000379710.1 SemiSWEET transporter -
N5O76_RS24050 (81258) 81258..82790 + 1533 WP_001017349.1 NAD(P)-binding domain-containing protein -
N5O76_RS24055 (82822) 82822..83274 - 453 WP_000670960.1 acyltransferase -
N5O76_RS24060 (83636) 83636..83866 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N5O76_RS24065 (83863) 83863..84279 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
N5O76_RS24070 (84354) 84354..85919 + 1566 WP_001128474.1 AAA family ATPase -
N5O76_RS24075 (85904) 85904..86926 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..182456 182456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T258389 WP_001034044.1 NZ_CP104540:83863-84279 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT258389 WP_001261286.1 NZ_CP104540:83636-83866 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References