Detailed information of TA system TA258380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3621539..3622376
• Replicon: chromosome
• Accession: NZ_CP104539
• Organism: Escherichia coli strain E6

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: N5O76_RS17730
• Protein ID: WP_000227784.1
• Coordinates: 3621834..3622376 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: N5O76_RS17725
• Protein ID: WP_001297137.1
• Coordinates: 3621539..3621850 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N5O76_RS17700 (3616559)	3616559..3617506	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
N5O76_RS17705 (3617528)	3617528..3619519	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
N5O76_RS17710 (3619509)	3619509..3620123	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
N5O76_RS17715 (3620123)	3620123..3620452	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
N5O76_RS17720 (3620464)	3620464..3621354	+	891	WP_000971336.1	heme o synthase	-
N5O76_RS17725 (3621539)	3621539..3621850	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
N5O76_RS17730 (3621834)	3621834..3622376	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
N5O76_RS17735 (3622432)	3622432..3623367	-	936	Protein_3470	sel1 repeat family protein	-
N5O76_RS17740 (3623775)	3623775..3625139	+	1365	WP_001000978.1	MFS transporter	-
N5O76_RS17745 (3625267)	3625267..3625758	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
N5O76_RS17750 (3625926)	3625926..3626837	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T258380 WP_000227784.1 NZ_CP104539:3621834-3622376 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T258380 NZ_CP104539:3621834-3622376 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT258380 WP_001297137.1 NZ_CP104539:3621539-3621850 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT258380 NZ_CP104539:3621539-3621850 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3