258371

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1085298..1085881 Replicon chromosome
Accession NZ_CP104539
Organism Escherichia coli strain E6

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag N5O76_RS05290 Protein ID WP_000254745.1
Coordinates 1085546..1085881 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag N5O76_RS05285 Protein ID WP_000581937.1
Coordinates 1085298..1085546 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O76_RS05275 (1081637) 1081637..1082938 + 1302 WP_021542211.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
N5O76_RS05280 (1082986) 1082986..1085220 + 2235 WP_000226815.1 GTP pyrophosphokinase -
N5O76_RS05285 (1085298) 1085298..1085546 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
N5O76_RS05290 (1085546) 1085546..1085881 + 336 WP_000254745.1 endoribonuclease MazF Toxin
N5O76_RS05295 (1085952) 1085952..1086743 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
N5O76_RS05300 (1086971) 1086971..1088608 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
N5O76_RS05305 (1088696) 1088696..1089994 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T258371 WP_000254745.1 NZ_CP104539:1085546-1085881 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT258371 WP_000581937.1 NZ_CP104539:1085298-1085546 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References