Detailed information of TA system TA025835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2224457..2225160
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TBSG_RS10710
• Protein ID: WP_003409778.1
• Coordinates: 2224831..2225160 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TBSG_RS21270
• Protein ID: WP_003409780.1
• Coordinates: 2224457..2224834 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS10665	2219615..2219806	+	192	WP_003409876.1	hypothetical protein	-
TBSG_RS10670	2219827..2220729	+	903	WP_003899097.1	hypothetical protein	-
TBSG_RS10675	2220740..2221090	+	351	WP_003899096.1	hypothetical protein	-
TBSG_RS10680	2221204..2221386	+	183	WP_003409870.1	hypothetical protein	-
TBSG_RS10685	2221379..2221780	-	402	WP_003409869.1	hypothetical protein	-
TBSG_RS10690	2221844..2222296	+	453	WP_003899095.1	lipoprotein	-
TBSG_RS10695	2222451..2223815	-	1365	WP_003903691.1	HNH endonuclease	-
TBSG_RS10700	2223870..2224460	+	591	WP_003409784.1	SEC-C domain-containing protein	-
TBSG_RS21270	2224457..2224834	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TBSG_RS10710	2224831..2225160	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TBSG_RS10715	2225370..2226140	-	771	WP_003409775.1	SDR family oxidoreductase	-
TBSG_RS10720	2226137..2227198	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TBSG_RS10725	2227195..2227710	-	516	WP_003409718.1	flavin reductase family protein	-
TBSG_RS10730	2227707..2228777	-	1071	WP_003899091.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T25835 WP_003409778.1 NC_016768:2224831-2225160 [Mycobacterium tuberculosis KZN 4207]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T25835 NC_016768:2224831-2225160 [Mycobacterium tuberculosis KZN 4207]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT25835 WP_003409780.1 NC_016768:2224457-2224834 [Mycobacterium tuberculosis KZN 4207]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT25835 NC_016768:2224457-2224834 [Mycobacterium tuberculosis KZN 4207]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8