Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4564745..4565242 Replicon chromosome
Accession NZ_CP104533
Organism Escherichia coli strain E10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5O77_RS22730 Protein ID WP_001173343.1
Coordinates 4564745..4564918 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag N5O77_RS22735 Protein ID WP_001312331.1
Coordinates 4564946..4565242 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O77_RS22720 (4561415) 4561415..4562872 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
N5O77_RS22725 (4563109) 4563109..4564626 + 1518 WP_000003806.1 lysine--tRNA ligase -
N5O77_RS22730 (4564745) 4564745..4564918 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5O77_RS22735 (4564946) 4564946..4565242 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5O77_RS22740 (4565470) 4565470..4565742 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5O77_RS22745 (4565754) 4565754..4565984 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
N5O77_RS22750 (4566165) 4566165..4567796 + 1632 WP_001216482.1 sensor histidine kinase -
N5O77_RS22755 (4567793) 4567793..4568512 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - papX 4493579..4565242 71663


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258339 WP_001173343.1 NZ_CP104533:c4564918-4564745 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT258339 WP_001312331.1 NZ_CP104533:c4565242-4564946 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References