Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1117352..1117935 Replicon chromosome
Accession NZ_CP104533
Organism Escherichia coli strain E10

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag N5O77_RS05380 Protein ID WP_000254750.1
Coordinates 1117600..1117935 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag N5O77_RS05375 Protein ID WP_000581937.1
Coordinates 1117352..1117600 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O77_RS05365 (1113691) 1113691..1114992 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
N5O77_RS05370 (1115040) 1115040..1117274 + 2235 WP_000226815.1 GTP pyrophosphokinase -
N5O77_RS05375 (1117352) 1117352..1117600 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
N5O77_RS05380 (1117600) 1117600..1117935 + 336 WP_000254750.1 endoribonuclease MazF Toxin
N5O77_RS05385 (1118007) 1118007..1118798 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
N5O77_RS05390 (1119026) 1119026..1120663 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
N5O77_RS05395 (1120751) 1120751..1122049 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T258320 WP_000254750.1 NZ_CP104533:1117600-1117935 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT258320 WP_000581937.1 NZ_CP104533:1117352-1117600 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References