Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4113837..4114413 Replicon chromosome
Accession NZ_CP104526
Organism Escherichia coli strain E8

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N5O73_RS20050 Protein ID WP_001297643.1
Coordinates 4114126..4114413 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag N5O73_RS20045 Protein ID WP_189915450.1
Coordinates 4113837..4114139 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O73_RS20030 (4110433) 4110433..4112583 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N5O73_RS20035 (4112633) 4112633..4112836 + 204 WP_000467859.1 YbdD/YjiX family protein -
N5O73_RS20040 (4112847) 4112847..4113803 + 957 WP_001393094.1 GTPase -
N5O73_RS20045 (4113837) 4113837..4114139 - 303 WP_189915450.1 BrnA antitoxin family protein Antitoxin
N5O73_RS20050 (4114126) 4114126..4114413 - 288 WP_001297643.1 BrnT family toxin Toxin
N5O73_RS20055 (4114612) 4114612..4115309 - 698 Protein_3924 IS1 family transposase -
N5O73_RS20060 (4115635) 4115635..4116867 + 1233 WP_016230806.1 multidrug efflux MFS transporter MdtM -
N5O73_RS20065 (4116908) 4116908..4118188 + 1281 WP_016230805.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(39-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T258310 WP_001297643.1 NZ_CP104526:c4114413-4114126 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11570.07 Da        Isoelectric Point: 9.7335

>AT258310 WP_189915450.1 NZ_CP104526:c4114139-4113837 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSYIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure

References