25830

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2184912..2185498 Replicon chromosome
Accession NC_016768
Organism Mycobacterium tuberculosis KZN 4207

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag TBSG_RS10430 Protein ID WP_003410010.1
Coordinates 2185154..2185498 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag TBSG_RS10425 Protein ID WP_003410014.1
Coordinates 2184912..2185160 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBSG_RS10405 2180895..2181662 - 768 WP_003410019.1 hemerythrin domain-containing protein -
TBSG_RS10410 2181819..2182175 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
TBSG_RS10415 2182228..2182500 + 273 WP_003410017.1 DUF1490 family protein -
TBSG_RS10420 2182497..2184812 + 2316 WP_003899120.1 cation transporter ATPase CptG -
TBSG_RS10425 2184912..2185160 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
TBSG_RS10430 2185154..2185498 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
TBSG_RS10435 2185587..2185922 + 336 WP_003410009.1 dehydrogenase -
TBSG_RS10440 2185823..2186080 - 258 WP_003410006.1 hypothetical protein -
TBSG_RS10445 2186166..2186507 + 342 WP_003410003.1 DUF2384 domain-containing protein -
TBSG_RS10450 2186504..2187064 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
TBSG_RS10455 2187584..2188123 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
TBSG_RS10460 2188349..2188777 - 429 WP_003906713.1 cellulose-binding protein -
TBSG_RS10465 2189193..2189792 - 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T25830 WP_003410010.1 NC_016768:2185154-2185498 [Mycobacterium tuberculosis KZN 4207]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T25830 NC_016768:2185154-2185498 [Mycobacterium tuberculosis KZN 4207]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT25830 WP_003410014.1 NC_016768:2184912-2185160 [Mycobacterium tuberculosis KZN 4207]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT25830 NC_016768:2184912-2185160 [Mycobacterium tuberculosis KZN 4207]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References