Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4578798..4579429 Replicon chromosome
Accession NZ_CP104523
Organism Escherichia coli strain E7

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag N5O80_RS22420 Protein ID WP_001260301.1
Coordinates 4579154..4579429 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID L4IZA1
Locus tag N5O80_RS22415 Protein ID WP_000593557.1
Coordinates 4578798..4579157 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O80_RS22390 (4574930) 4574930..4575874 + 945 WP_000947063.1 nickel ABC transporter permease subunit NikB -
N5O80_RS22395 (4575871) 4575871..4576704 + 834 WP_021539320.1 nickel ABC transporter permease subunit NikC -
N5O80_RS22400 (4576704) 4576704..4577468 + 765 WP_001136206.1 nickel import ATP-binding protein NikD -
N5O80_RS22405 (4577465) 4577465..4578271 + 807 WP_000173650.1 nickel import ATP-binding protein NikE -
N5O80_RS22410 (4578277) 4578277..4578678 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
N5O80_RS22415 (4578798) 4578798..4579157 - 360 WP_000593557.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N5O80_RS22420 (4579154) 4579154..4579429 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
N5O80_RS22425 (4579502) 4579502..4580626 - 1125 WP_001318088.1 ABC transporter permease -
N5O80_RS22430 (4580626) 4580626..4583361 - 2736 WP_000149178.1 ribosome-associated ATPase/putative transporter RbbA -
N5O80_RS22435 (4583358) 4583358..4584425 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4546316..4583361 37045


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T258285 WP_001260301.1 NZ_CP104523:c4579429-4579154 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13389.28 Da        Isoelectric Point: 5.1987

>AT258285 WP_000593557.1 NZ_CP104523:c4579157-4578798 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB L4IZA1

References