Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2093333..2093969 Replicon chromosome
Accession NC_016768
Organism Mycobacterium tuberculosis KZN 4207

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag TBSG_RS10035 Protein ID WP_003410654.1
Coordinates 2093333..2093743 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag TBSG_RS10040 Protein ID WP_003410651.1
Coordinates 2093736..2093969 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBSG_RS10015 2088930..2090153 + 1224 WP_003901321.1 class I SAM-dependent methyltransferase -
TBSG_RS10020 2090099..2091625 - 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
TBSG_RS10025 2091622..2092248 - 627 WP_003410658.1 precorrin-8X methylmutase -
TBSG_RS10030 2092258..2093349 - 1092 WP_003900454.1 precorrin-3B synthase -
TBSG_RS10035 2093333..2093743 - 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
TBSG_RS10040 2093736..2093969 - 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
TBSG_RS10045 2094047..2097631 + 3585 WP_003410648.1 cobaltochelatase subunit CobN -
TBSG_RS10050 2097715..2098119 + 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T25827 WP_003410654.1 NC_016768:c2093743-2093333 [Mycobacterium tuberculosis KZN 4207]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T25827 NC_016768:c2093743-2093333 [Mycobacterium tuberculosis KZN 4207]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT25827 WP_003410651.1 NC_016768:c2093969-2093736 [Mycobacterium tuberculosis KZN 4207]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT25827 NC_016768:c2093969-2093736 [Mycobacterium tuberculosis KZN 4207]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References