Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 477152..477987 Replicon chromosome
Accession NZ_CP104521
Organism Escherichia coli strain E5

Toxin (Protein)


Gene name yeeV Uniprot ID V0Z9U3
Locus tag N5O78_RS02280 Protein ID WP_000854750.1
Coordinates 477610..477987 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag N5O78_RS02275 Protein ID WP_001278232.1
Coordinates 477152..477520 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O78_RS02240 (472951) 472951..473631 + 681 WP_094122466.1 WYL domain-containing protein -
N5O78_RS02245 (473779) 473779..474456 + 678 WP_094122412.1 hypothetical protein -
N5O78_RS02250 (474462) 474462..474695 + 234 WP_001278287.1 DUF905 family protein -
N5O78_RS02255 (474794) 474794..475612 + 819 WP_077737895.1 DUF932 domain-containing protein -
N5O78_RS02260 (475704) 475704..476189 + 486 WP_000206658.1 antirestriction protein -
N5O78_RS02265 (476205) 476205..476681 + 477 WP_001367707.1 RadC family protein -
N5O78_RS02270 (476768) 476768..476989 + 222 WP_000691819.1 DUF987 domain-containing protein -
N5O78_RS02275 (477152) 477152..477520 + 369 WP_001278232.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
N5O78_RS02280 (477610) 477610..477987 + 378 WP_000854750.1 TA system toxin CbtA family protein Toxin
N5O78_RS02285 (477984) 477984..478472 + 489 WP_000761685.1 DUF5983 family protein -
N5O78_RS02290 (478484) 478484..478681 + 198 WP_000839280.1 DUF957 domain-containing protein -
N5O78_RS02295 (478766) 478766..478909 + 144 Protein_441 hypothetical protein -
N5O78_RS02300 (479865) 479865..481901 + 2037 WP_000417002.1 hypothetical protein -
N5O78_RS02305 (482300) 482300..482479 - 180 Protein_443 peptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE 460721..490210 29489


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 13919.90 Da        Isoelectric Point: 8.2905

>T258238 WP_000854750.1 NZ_CP104521:477610-477987 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp


Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT258238 WP_001278232.1 NZ_CP104521:477152-477520 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0Z9U3


Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References