Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 279041..279538 Replicon chromosome
Accession NZ_CP104521
Organism Escherichia coli strain E5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5O78_RS01300 Protein ID WP_001173343.1
Coordinates 279365..279538 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag N5O78_RS01295 Protein ID WP_001317561.1
Coordinates 279041..279337 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O78_RS01275 (275773) 275773..276492 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5O78_RS01280 (276489) 276489..278120 - 1632 WP_001216477.1 sensor histidine kinase -
N5O78_RS01285 (278300) 278300..278530 + 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
N5O78_RS01290 (278542) 278542..278814 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5O78_RS01295 (279041) 279041..279337 + 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5O78_RS01300 (279365) 279365..279538 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5O78_RS01305 (279657) 279657..281174 - 1518 WP_000003805.1 lysine--tRNA ligase -
N5O78_RS01310 (281411) 281411..282868 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258237 WP_001173343.1 NZ_CP104521:279365-279538 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT258237 WP_001317561.1 NZ_CP104521:279041-279337 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References