Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1778..2325 Replicon plasmid p2
Accession NZ_CP104518
Organism Escherichia coli strain E4

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag N5O79_RS25050 Protein ID WP_001384452.1
Coordinates 2047..2325 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag N5O79_RS25045 Protein ID WP_000079941.1
Coordinates 1778..2047 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O79_RS25035 (N5O79_25035) 1..873 + 873 WP_021532687.1 incFII family plasmid replication initiator RepA -
N5O79_RS25040 (N5O79_25040) 1565..1723 + 159 WP_000841008.1 hypothetical protein -
N5O79_RS25045 (N5O79_25045) 1778..2047 + 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
N5O79_RS25050 (N5O79_25050) 2047..2325 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
N5O79_RS25055 (N5O79_25055) 2365..3213 + 849 WP_001057996.1 3'-5' exonuclease -
N5O79_RS25060 (N5O79_25060) 3450..3575 - 126 Protein_5 MerR family transcriptional regulator -
N5O79_RS25065 (N5O79_25065) 3570..4184 + 615 Protein_6 transposase -
N5O79_RS25070 (N5O79_25070) 4193..5053 + 861 WP_000493383.1 EamA family transporter -
N5O79_RS25075 (N5O79_25075) 5085..6284 - 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
N5O79_RS25080 (N5O79_25080) 6390..7040 + 651 WP_000164043.1 tetracycline resistance transcriptional repressor TetR(A) -
N5O79_RS25085 (N5O79_25085) 7072..7314 - 243 WP_000844627.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul1 / qacE / aac(3)-VIa / ant(3'')-Ia / blaCMY-2 htpB 1..119377 119377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T258236 WP_001384452.1 NZ_CP104518:2047-2325 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT258236 WP_000079941.1 NZ_CP104518:1778-2047 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References